Abstract:

Hyperthermia during radiofrequency ablation causes reversible and irreversible changes of the electrophysiological properties of cardiac tissue. However, the mechanisms are incompletely understood. We studied changes of conduction velocity (CV) in rat myocardium under hyperthermic conditions from macroscopic to microscopic scale by using simultaneous optical mapping and a miniaturized electrode array. Atrial preparations from five rats were superfused at tissue bath temperatures between 36.7°C and 43.8°C. Optical mapping data showed an elevated median CV by 21% when increasing the temperature from 36.7°C to 42.0°C. CV did not increase above 42.0°C. Electrical measurements revealed a similar temperature dependence of CV between 36.7°C and 42.0°C, i.e. an increase of median CV by 26%. The consolidation of optical and electrical data in this study allowed investigation of excitation during global hyperthermia. Macroscopic optical mapping and microscopic electrical measurements demonstrated that hyperthermia strongly influenced electrical propagation at a microscopic scale.