Abstract:

Local brain hypothermia is an attractive method for providing cerebral neuroprotection for ischemic stroke patients and at the same time reducing systemic side effects of cooling. In acute ischemic stroke patients with large vessel occlusion, combination with endovascular mechanical recanalization treatment could potentially allow for an alleviation of inflammatory and apoptotic pathways in the critical phase of reperfusion. The direct cooling of arterial blood by means of an intra-carotid heat exchange catheter compatible with recanalization systems is a novel promising approach. Focusing on the concept of "cold reperfusion", we developed an energetic model to calculate the rate of temperature decrease during intra-carotid cooling in case of physiological as well as decreased perfusion. Additionally, we discussed and considered the effect and biological significance of temperature decrease on resulting brain perfusion. Our model predicted a 2 °C brain temperature decrease in 8.3, 11.8 and 26.2 min at perfusion rates of 50, 30 and 10ml100g⋅min, respectively. The systemic temperature decrease - caused by the venous blood return to the main circulation - was limited to 0.5 °C in 60 min. Our results underline the potential of catheter-assisted, intracarotid blood cooling to provide a fast and selective brain temperature decrease in the phase of vessel recanalization. This method can potentially allow for a tissue hypothermia during the restoration of the physiological flow and thus a "cold reperfusion" in the setting of mechanical recanalization.

Abstract:

Atypical atrial flutter (AFlut) is a reentrant arrhythmia which patients frequently develop after ablation for atrial fibrillation (AF). Indeed, substrate modifications during AF ablation can increase the likelihood to develop AFlut and it is clinically not feasible to reliably and sensitively test if a patient is vulnerable to AFlut. Here, we present a novel method based on personalized computational models to identify pathways along which AFlut can be sustained in an individual patient. We build a personalized model of atrial excitation propagation considering the anatomy as well as the spatial distribution of anisotropic conduction velocity and repolarization characteristics based on a combination of a priori knowledge on the population level and information derived from measurements performed in the individual patient. The fast marching scheme is employed to compute activation times for stimuli from all parts of the atria. Potential flutter pathways are then identified by tracing loops from wave front collision sites and constricting them using a geometric snake approach under consideration of the heterogeneous wavelength condition. In this way, all pathways along which AFlut can be sustained are identified. Flutter pathways can be instantiated by using an eikonal-diffusion phase extrapolation approach and a dynamic multifront fast marching simulation. In these dynamic simulations, the initial pattern eventually turns into the one driven by the dominant pathway, which is the only pathway that can be observed clinically. We assessed the sensitivity of the flutter pathway maps with respect to conduction velocity and its anisotropy. Moreover, we demonstrate the application of tailored models considering disease-specific repolarization properties (healthy, AF-remodeled, potassium channel mutations) as well as applicabiltiy on a clinical dataset. Finally, we tested how AFlut vulnerability of these substrates is modulated by exemplary antiarrhythmic drugs (amiodarone, dronedarone). Our novel method allows to assess the vulnerability of an individual patient to develop AFlut based on the personal anatomical, electrophysiological, and pharmacological characteristics. In contrast to clinical electrophysiological studies, our computational approach provides the means to identify all possible AFlut pathways and not just the currently dominant one. This allows to consider all relevant AFlut pathways when tailoring clinical ablation therapy in order to reduce the development and recurrence of AFlut.

Abstract:

Each heartbeat is initiated by cyclic spontaneous depolarization of cardiomyocytes in the sinus node forming the primary natural pacemaker. In patients with end-stage renal disease undergoing hemodialysis, it was recently shown that the heart rate drops to very low values before they suffer from sudden cardiac death with an unexplained high incidence. We hypothesize that the electrolyte changes commonly occurring in these patients affect sinus node beating rate and could be responsible for severe bradycardia. To test this hypothesis, we extended the Fabbri et al. computational model of human sinus node cells to account for the dynamic intracellular balance of ion concentrations. Using this model, we systematically tested the effect of altered extracellular potassium, calcium, and sodium concentrations. Although sodium changes had negligible (0.15 bpm/mM) and potassium changes mild effects (8 bpm/mM), calcium changes markedly affected the beating rate (46 bpm/mM ionized calcium without autonomic control). This pronounced bradycardic effect of hypocalcemia was mediated primarily by I attenuation due to reduced driving force, particularly during late depolarization. This, in turn, caused secondary reduction of calcium concentration in the intracellular compartments and subsequent attenuation of inward I and reduction of intracellular sodium. Our in silico findings are complemented and substantiated by an empirical database study comprising 22,501 pairs of blood samples and in vivo heart rate measurements in hemodialysis patients and healthy individuals. A reduction of extracellular calcium was correlated with a decrease of heartrate by 9.9 bpm/mM total serum calcium (p < 0.001) with intact autonomic control in the cross-sectional population. In conclusion, we present mechanistic in silico and empirical in vivo data supporting the so far neglected but experimentally testable and potentially important mechanism of hypocalcemia-induced bradycardia and asystole, potentially responsible for the highly increased and so far unexplained risk of sudden cardiac death in the hemodialysis patient population.

Abstract:

Chronic kidney disease (CKD) affects 13% of the worldwide population and end stage patients often receive haemodialysis treatment to control the electrolyte concentrations. The cardiovascular death rate increases by 10% - 30% in dialysis patients than in general population. To analyse possible links between electrolyte concentration variation and cardiovascular diseases, a continuous non-invasive monitoring tool enabling the estimation of potassium and calcium concentration from features of the ECG is desired. Although the ECG was shown capable of being used for this purpose, the method still needs improvement. In this study, we examine the influence of lead reduction techniques on the estimation results of serum calcium and potassium concentrations.We used simulated 12 lead ECG signals obtained using an adapted Himeno et al. model. Aiming at a precise estimation of the electrolyte concentrations, we compared the estimation based on standard ECG leads with the estimation using linearly transformed fusion signals. The transformed signals were extracted from two lead reduction techniques: principle component analysis (PCA) and maximum amplitude transformation (Max- Amp). Five features describing the electrolyte changes were calculated from the signals. To reconstruct the ionic concentrations, we applied a first and a third order polynomial regression connecting the calculated features and concentration values. Furthermore, we added 30 dB white Gaussian noise to the ECGs to imitate clinically measured signals. For the noisefree case, the smallest estimation error was achieved with a specific single lead from the standard 12 lead ECG. For example, for a first order polynomial regression, the error was 0.0003±0.0767 mmol/l (mean±standard deviation) for potassium and -0.0036±0.1710 mmol/l for calcium (Wilson lead V1). For the noisy case, the PCA signal showed the best estimation performance with an error of -0.003±0.2005 mmol/l for potassium and -0.0002±0.2040 mmol/l for calcium (both first order fit). Our results show that PCA as ECG lead reduction technique is more robust against noise than MaxAmp and standard ECG leads for ionic concentration reconstruction.

Abstract:

Changes of serum and extracellular ion concentrations occur regularly in patients with chronic kidney disease (CKD). Recently, hypocalcemia, i.e. a decrease of the extra-cellular calcium concentration [Ca2+]o, has been suggested as potential pathomechanism contributing to the unexplained high rate of sudden cardiac death (SCD) in CKD patients. In particular, there is a hypothesis that hypocalcaemia could slow down natural pacemaking in the human sinus node to fatal degrees. Here, we address the question whether there are inter-species differences in the response of cellular sinus node pacemaking to changes of [Ca2+]o. Towards this end, we employ computational models of mouse, rabbit and human sinus node cells. The Fabbri et al. human model was updated to consider changes of intracellular ion concentrations. We identified crucial inter-species differences in the response of cellular pacemaking in the sinus node to changes of [Ca2+]o with little changes of cycle length in mouse and rabbit models (<83 ms) in contrast to a pronounced bradycardic effect in the human model (up to > 1000 ms). Our results suggest that experiments with human sinus node cells are required to investigate the potential mechanism of hypocalcaemia-induced bradycardic SCD in CKD patients and small animal models are not well suited.

Abstract:

Atrial fibrillation (AF) is the most prevalent form of cardiac arrhythmia. The atrial wall thickness (AWT) can potentially improve our understanding of the mechanism underlying atrial structure that drives AF and provides important clinical information. However, most existing studies for estimating AWT rely on ruler-based measurements performed on only a few selected locations in 2D or 3D using digital calipers. Only a few studies have developed automatic approaches to estimate the AWT in the left atrium, and there are currently no methods to robustly estimate the AWT of both atrial chambers. Therefore, we have developed a computational pipeline to automatically calculate the 3D AWT across bi-atrial chambers and extensively validated our pipeline on both ex vivo and in vivo human atria data. The atrial geometry was first obtained by segmenting the atrial wall from the MRIs using a novel machine learning approach. The epicardial and endocardial surfaces were then separated using a multi-planar convex hull approach to define boundary conditions, from which, a Laplace equation was solved numerically to automatically separate bi-atrial chambers. To robustly estimate the AWT in each atrial chamber, coupled partial differential equations by coupling the Laplace solution with two surface trajectory functions were formulated and solved. Our pipeline enabled the reconstruction and visualization of the 3D AWT for bi-atrial chambers with a relative error of 8% and outperformed existing algorithms by >7%. Our approach can potentially lead to improved clinical diagnosis, patient stratification, and clinical guidance during ablation treatment for patients with AF.

Abstract:

Under persistent atrial fibrillation (peAF), cardiac tissue experiences electrophysiological and structural remodeling. Fibrosis in the atrial tissue has an important impact on the myocyte action potential and its propagation. The objective of this work is to explore the effect of heterogeneities present in the fibrotic tissue and their impact on the intracardiac electrogram (EGM). Human atrial myocyte and fibroblast electrophysiology was simulated using mathematical models proposed by Koivumäki et al. to represent electrical remodeling under peAF and the paracrine effect of the transforming grow factor 1 (TGF-1). 2D tissue simulations were computed varying the density of fibrosis (10%, 20% and 40%), myofibroblasts and collagen were randomly distributed with different ratios (0%-100%, 50%-50% and 100%- 0%). Results show that increasing the fibrosis density changes the re-entry dynamics from functional to anatomical due to a block in conduction in regions with high fibrosis density (40%). EGM morphology was affected by different ratios of myofibroblasts-collagen. For low myofibroblast densities (below 50%) the duration of active segments was shorter compared to higher myofibroblasts densities (above 50%). Our results show that fibrosis heterogeneities can alter the dynamics of the re-entry and the morphology of the EGM.

Abstract:

The SuLMaSS project [1] will advance, develop, build, evaluate, and test infrastructure for sustainable lifecycle management of scientific software. The infrastructure is tested and evaluated by an existing cardiac electrophysiology simulation software project, which is currently in the prototype state and will be advanced towards optimal usability and a large and active user community. Thus, SuLMaSS is focused on designing and implementing application-oriented e-research technologies and the impact is three-fold: - Provision of a high quality, user-friendly cardiac electrophysiology simulation software package that accommodates attestable needs of the scientific community. - Delivery of infrastructure components for testing, safe-keeping, referencing, and versioning of all phases of the lifecycle of scientific software. - Serve as a best practice example for sustainable scientific software management. Scientific software development in Germany and beyond shall benefit through both the aforementioned best practice role model and the advanced infrastructure that will, in part, be available for external projects as well. With adding value for the wider scientific cardiac electrophysiology community, the software will be available under an open source license and be provided for a large share of people and research groups that can potentially leverage computational cardiac modeling methods. Institutional infrastructure will be extended to explore, evaluate and establish the basis for research software development regarding testing, usage, maintenance and support. The cardiac electrophysiology simulator will drive and showcase the infrastructure formation, thus serving as a lighthouse project. The developed infrastructure can be used by other scientific software projects in future and aims to support the full research lifecycle from exploration through conclusive analysis and publication, to archival, and sharing of data and source code, thus increasing the quality of research results. Moreover it will foster a community-based collaborative development and improve sustainability of research software.

Abstract:

The heart acts as the pump of the cardiovascular system due to the active stress developed in individ- ual cardiac muscle cells. The spatio-temporal distribution of this active stress could contain relevant diagnostic information but can currently not be measured in vivo. We introduce a method to esti- mate dynamic cardiac active stress fields from endocardial surface motion tracking derived from e.g. magnetic resonance imaging data. This ill-posed non-linear problem is solved using Tikhonov regu- larization in space and time in conjunction with a continuum mechanics forward model. We present a proof-of-concept using data from a biophysically detailed multiscale model of cardiac electrome- chanics (7649 tetrahedral elements) in which we could accurately reproduce cardiac motion (surface error <0.4 mm) and identify non-contracting regions due to myocardial infarction scars (active stress error <10 kPa). This inverse method could eventually be used to non-invasively derive personalized diagnostic information in terms of dynamic active stress fields which are not accessible today.

Abstract:

Heterogeneous atrial substrate can induce, maintain and promote cardiac arrhythmias. The level of heterogeneity may be used to assess disease progression. One key parameter, suspected to be correlated with tissue vitality is the conduction velocity (CV). By measuring not only the current CV of the patient but rather its rate dependent changes, restitution information is gained. In the following, we show our approach towards a patient-specific quantitative atrial substrate characterization by combining sets of local and global CV restitution measurements to create a parametrization of the individual patient substrate characteristics.

Abstract:

Stroke is the third-most cause of death in developed countries. A new promising treatment method in case of an ischemic stroke is selective intracarotid blood cooling combined with mechanical artery recanalization. However, the control of the treatment requires invasive or MRI-assisted measurement of cerebral temperature. An auspicious alternative is the use of computational modeling. In this work, we extended an existing 1D hemodynamics model including the characteristics of the anterior, middle and posterior cerebral artery. Furthermore, seven ipsilateral anastomoses were additionally integrated for each hemisphere. A potential stenosis was placed into the M1 segment of the middle cerebral artery, due to the highest risk of occlusion there. The extended model was evaluated for various degrees of collateralization (“poor”, “partial” and “good”) and degrees of stenosis (0%, 50%, 75% and 99.9%). Moreover, cerebral autoregulation was considered in the model. The higher the degree of collateralization and the degree of stenosis, the higher was the blood flow through the collaterals. Hence, a patient with a good collateralization could compensate a higher degree of occlusion and potentially has a better outcome after an ischemic stroke. For a 99.9% stenosis, an increased summed mean blood flow through the collaterals of +97.7% was predicted in case of good collateralization. Consequently, the blood supply via the terminal branches of the middle cerebral artery could be compensated up to 44.4% to the physiological blood flow. In combination with a temperature model, our model of the cerebral collateral circulation can be used for tailored temperature prediction for patients to be treated with selective therapeutic hypothermia.

Abstract:

In Western countries, stroke is the third-most cause of death; 35- 55% of the survivors experience permanent disability. Mild therapeutic hypothermia (TH) showed neuroprotective effect in patients returning from cardiac arrest and is therefore assumed to decrease stroke induced cerebral damage. Recently, an intracarotid cooling sheath was developed to induce local TH in the penumbra using the cooling effect of cerebral blood flow via collaterals. Computational modeling provides unique opportunities to predict the resulting cerebral temperature without invasive procedures. In this work, we generated a simplified brain model to establish a cerebral temperature calculation using Pennes’ bio-heat equation and a 1D hemodynamics model of the cranial artery tree. In this context, we performed an extensive literature research to assign the terminal segments of the latter to the corresponding perfused tissue. Using the intracarotid cooling method, we simulated the treatment with TH for different degrees of stenosis in the middle cerebral artery (MCA) and analyzed the resulting temperature spatialtemporal distributions of the brain and the systemic body considering the influence of the collaterals on the effect of cooling.

Abstract:

Changes of serum and extracellular ion concentrations occur regularly in patients with chronic kidney disease (CKD). Recently, hypocalcaemia, i.e. a decrease of the extracellular calcium concentration [Ca 2+ ] o , has been suggested as potential pathomechanism contributing to the unexplained high rate of sudden cardiac death (SCD) in CKD patients. In particular, there is a hypothesis that hypocalcaemia could slow down natural pacemaking in the human sinus node to fatal degrees. Here, we address the question whether there are inter-species differences in the response of cellular sinus node pacemaking to changes of [Ca 2+ ] o . Towards this end, we employ computational models of mouse, rabbit and human sinus node cells. The Fabbri et al. human model was updated to consider changes of intracellular ion concentrations. We identified crucial inter-species differences in the response of cellular pacemaking in the sinus node to changes of [Ca 2+ ] o with little changes of cycle length in mouse and rabbit models (<83 ms) in contrast to a pronounced bradycardic effect in the human model (up to >1000 ms). Our results suggest that experiments with human sinus node cells are required to investigate the potential mechanism of hypocalcaemia-induced bradycardic SCD in CKD patients and small animal models are not well suited.

Abstract:

In Western countries, stroke is the third-most widespread cause of death. 80% of all strokes are ischemic and show a mortality rate of about 25%. Furthermore, 35-55% of affected patients retain a permanent disability. Therapeutic hypothermia (TH) could decrease inflammatory processes and the stroke-induced cerebral damage. Currently, the standard technique to induce TH is cooling of the whole body, which can cause several side effects. A novel cooling sheath uses intra-carotid blood cooling to induce local TH. Unfortunately, the control of the temporal and spatial cerebral temperature course requires invasive temperature measurements. Computational modeling could be used to predict the resulting temperature courses instead. In this work, a detailed 1D hemodynamics model of the cerebral arterial system was coupled with an energetic temperature model. For physiological conditions, 50% and 100% M1-stenoses, the temperatures in the supply area of the middle cerebral artery (MCA) and of the systemic body was analyzed. A 2K temperature decrease was reached within 10min of cooling for physiological conditions and 50% stenosis. For 100% stenosis, a significant lower cooling effect was observed, resulting in a maximum cerebral temperature decrease of 0.7K after 30min of cooling. A significant influence of collateral flow rates on the cooling effect was observed. However, regardless of the stenosis degree, the temperature decrease was strongest within the first 20min of cooling, which demonstrates the fast and effective impact of intra-carotid blood cooling.

Abstract:

In Europe, the prevalence of chronic kidney disease lay at approximately 18.38% in 2016. A common treatment for patients in the end stage of this disease is haemodialysis. However, patients undergoing this therapy suffer from an increased risk of cardiac death. A hypothesis is that the cause is an inbalanced electrolyte concentration. To study the underlying mechanisms of this phenomenon and fight the consequences, a continous non-invasive monitoring technique is desired. In this work, we investigated the possibility to reconstruct the extracellular concentrations of potassium and calcium from ECG signals. Therefore, we extracted 71 ECGs using the simulation results of a modified Himeno et al. ventricular cell model comprising variations of the extracellular ionic concentrations of potassium and calcium. The changes dependent on the different extracellular ionic concentrations were captured with five ECG features. These were used to train an artificial neural network for regression. The study was performed both for noise-free and noisy data. The estimation error for the reconstruction of the potassium concentrations was -0.01±0.14 mmol/l (mean±standard deviation) in the noise- free case, -0.03±0.46mmol/l in the noisy case (30dB SNR). For calcium, the result was 0.01±0.11mmol/l in the noise- free case, 0.02±0.17mmol/l in the noisy case. For both ion types, the result was improved by augmenting the dataset. We therefore conclude that with the calculated features, we are able to reconstruct the extracellular ionic concentrations for both potassium and calcium with an acceptable precision. When analysing noisy signals, the accuracy of the estimation method is still sufficient but can be further improved by an augmentation of the dataset.

Abstract:

The risk of sudden cardiac death (SCD) is increased 14-fold in chronic hemodialysis (HD) patients compared to patients with normal kidney function suffering from cardiovascular diseases. This high rate is not explained by traditional cardiovascular risk factors. Recently, severe bradycardia has been implicated in SCD in HD patients. Mathematical modelling suggests an electrophysiological link between low serum calcium (Ca) levels and bradycardia. Therefore, we analyzed the correlation between heart rate (HR) and Ca as well as potassium (K).

Abstract:

The outcomes of ablation targeting either reentry activations or fractionated activity during persistent atrial fibrillation (AF) therapy remain suboptimal due to, among others, the intricate underlying AF dynamics. In the present work, we sought to investigate such AF dynamics in a heterogeneous simulation setup using recurrence quantification analysis (RQA). AF was simulated in a spherical model of the left atrium, from which 412 unipolar atrial electrograms (AEGs) were extracted (2 s duration; 5 mm spacing). The phase was calculated using the Hilbert transform, followed by the identification of points of singularity (PS). Three regions were defined according to the occurrence of PSs: 1) no rotors; 2) transient rotors and; 3) long-standing rotors. Bipolar AEGs (1114) were calculated from pairs of unipolar nodes and bandpass filtered (30-300 Hz). The CARTO criterion (Biosense Webster) was used for AEGs classification (normal vs. fractionated). RQA attributes were calculated from the filtered bipolar AEGs: determinism (DET); recurrence rate (RR); laminarity (LAM). Sample entropy (SampEn) and dominant frequency (DF) were also calculated from the AEGs. Regions with longstanding rotors have shown significantly lower RQA attributes and SampEn when compared to the other regions, suggesting a higher irregular behaviour (P≤0.01 for all cases). Normal and fractionated AEGs were found in all regions (respectively; Region 1: 387 vs. 15; Region 2: 221 vs. 13; Region 3: 415 vs. 63). Region 1 vs. Region 3 have shown significant differences in normal AEGs (P≤0.0001 for all RQA attributes and SampEn), and significant differences in fractionated AEGs for LAM, RR and SampEn (P=0.0071, P=0.0221 and P=0.0086, respectively). Our results suggest the co-existence of normal and fractionated AEGs within long-standing rotors. RQA has unveiled distinct dynamic patterns–irrespective of AEGs classification–related to regularity structures and their nonstationary behaviour in a rigorous deterministic context.

Abstract:

Intracardiac electrograms (EGMs) form the basis for the diagnosis of arrhythmia mechanisms. Bipolar EGMs dominate clinical practice despite major disadvantages over unipolar EGMs since noise strongly distorts the latter. In this study, we quantified and reduced the noise level of uni- and bipolar EGMs recorded with Rhythmia HDx and the Orion catheter. Distinct noise frequencies in the power spectral density (PSD) were detected with a sliding win- dow of variable width and subsequently removed by notch filtering. The absolute peak to peak voltage remaining in the inactive segments after baseline removal quantified the noise level of the system. An international, multi-center selection of 33 patients served as a broad sample cohort. The case-specific detection and removal of noise peaks reduced the noise level in unipolar EGMs by 30% to 0.076 mV compared to standard clinical filtering. With a bipolar noise level of 0.01 mV, we saw that Rhythmia HDx meets the low noise floor claimed in the system specifica- tions. Certain noise frequencies presented permanently in all cases whereas others showed up only intermittently or in individual cases. The suggested extension of filter settings lowers the noise level, enhances the detailed segmentation of low volt- age areas, and encourages to exploit the advantages of unipolar over bipolar EGMs in clinical practice.

Abstract:

Improved understanding of the effects of variability in electrophysiological activity within the human heart is key to understanding and predicting cardiovascular response to disease and treatments. Previous studies have considered either regional variation in action potentials or inter-subject variability within a single region of the atria. In this study, we hypothesize that the regional differences in morphology derive not only from variation in dependence on individual conductances, but also from the relationship between multiple conductances. Using the Monte-Carlo Sampling Method and the Maleckar cellular model for electrophysiology, we created an In-Silico Population of Models. Each conductance was varied +/- 100% from the standard model. The population was divided into regional groups based on biomarkers. Results showed regional variation in the dependence on relationships between conductances. In the right atrial appendage the value of gK1 was found to be only twice as influential as the relationship between gK1 and gKur on the APD90 biomarker. Other relationships that had a significant impact included gTo-gKur; gKr-gK1; gNaK- gNaCa and gKur-gNaK for various regions. R2 values for first order linear regression models showed significant relationships were left out in the analysis. This was significantly improved in the second order R2 values.

Abstract:

Several works have studied arrhythmogenicity of a given atrial model using different methods to initiate and simulate the perpetuation of re-entrant activity. We evaluated and compared two state-of-the-art methods showing their influence on the estimated vulnerability to arrhythmia of an atrial model.

Abstract:

In silico studies are often used to analyze mechanisms of cardiac arrhythmias. The electrophysiological cell models that are used to simulate the membrane potential in these studies range from highly detailed physiological models to simplistic phenomenological models. To effectively cover the middle ground between those cell models, we utilize the manifold boundary approxi- mation method (MBAM) to systematically reduce the widely used O’Hara-Rudy ventricular cell model (ORd) and investigate the influence of parametrization of the model as well as different strategies of choosing input quantities, further called quantities of interest (QoI). As a result of the reduction process, we present three re- duced model variants of the ORd model that only contain a fraction of the original model’s ionic currents resulting in a twofold speedup in computation times compared to the original model. We find that the reduced models show similar action potential duration restitution and repolarization rates. Additionally, we are able to initialize and observe stable spiral wave dynamics on a 3D tissue patch for 2 out of the 3 reduced models.

Abstract:

Catheter ablation targeting low voltage areas (LVA) is commonly being used to treat atrial fibrillation (AF) in pa- tients with persistent AF. However, it is not always certain that the areas marked as low voltage (LV) are correct. This can be related to how the voltage is calculated. There- fore, this paper focuses on comparing different calculation methods, specifically, with regards to spatial distribution. Two voltage maps obtained in AF were used, removing points which did not meet the required specifications. The peaks for the remaining points, in regions of the left atrium, were then found and the voltage was calculated based on taking the peak to peak (p2p) for different beats. For around 30% of the points on the map, the voltage only changed by 0.1mV when taking one beat versus all beats. However, for some individual points, the difference was substantial, around 0.8mV, depending on the beat cho- sen. Additionally, the inter-method variability increased by around 0.1mV when considering all methods compared to only methods calculated using more than one point. It was found that taking a method which considers all p2p values would be a more appropriate method for cal- culating the voltage. Thus, providing a technique, which could improve the accuracy of identifying LVA in an AF map.

Abstract:

Nowadays, a large share of the global population is affected by heart rhythm disorders. Computational modelling is a useful tool for understanding the dynamics of cardiac arrhythmias. Several recent clinical and experimental studies suggest that atrial fibrillation is maintained by re-entrant drivers (e.g. rotors). As a consequence, numerous works have addressed atrial arrhythmogenicity of a given electrophysiological model using different methods to simulate the perpetuation of re-entrant activity. However, no common procedure to test atrial fibrillation vulnerability has yet been defined. Here, we systematically evaluate and compare two state-of-the-art methods. The first one is rapid extrastimulus pacing from rim of the four pulmonary veins. The second consists of placing phase singularities in the atria, estimating an activation time map by solving the Eikonal equation and finally using this as initial condition for the electrical cardiac propagation simulation. In this way, we are forcing the wavefronts to follow re-entrant circuits with low computational cost thus less simulation time. We aim to identify a methodology to quantify arrhythmia vulnerability on patient-specific atrial geometries and substrates. We will proceed with in-silico experiments, comparing the results of these two methods to initiate re-entrant activity, checking the influence of the different parameters on the dynamics on the re-entrant drivers and finally extracting a valid set of parameters allowing to reliably assess re-entry vulnerability. The final objective is to come up with an easily reproducible minimal set of simulations to assess vulnerability of a particular atrial substrate (cellular and tissue model) or of distinct anatomical atrial geometries to arrhythmic episodes. Given the great need of exploring susceptibility to atrial arrhythmias, i.e. after a first ablation procedure, this study can provide a useful tool to test new treatment strategies and to learn how to prevent the onset and progression of atrial fibrillation.

Abstract:

Atrial fibrillation is the most common cardiac arrhythmia characterized by a rapid and irregular atrial excitation rate. Mimicking this behaviour, the S1-S2 stimulation protocol is currently the clinically established method for measuring tissue rate dependency, leading to a need for an automated segmentation method. We propose a method for stimulus artefact removal tailored towards the S1-S2 protocol. We show that this method results in the detection of atrial signals minimizing distortion by the stimulus artefact and is therefore an effective segmentation tool and a building block for automation of signal analysis.