Abstract:

The Subject of this work is producing the phantoms used for investigation of contrast image in Confocal Laser Endomicroscopy (CLE). Created phantoms should simulate the simplified cellular structure of human tissue after injection with fluorescein sodium (FS). FS is diffused into the intracellular and extracellular fluids but not into cell nuclei. Phantom would have fluorescent matrix (simulating these fluids) and glass particles (simulating cell nuclei). Several experiments have been performed in order to research the best production method - including both process and used materials for matrix base of the samples - in order to continue further investigation. There were two production methods, based on different materials for matrix base - elastomer and acrylate. Chosen phantoms were optically characterized with help of spectrophotometer. All of the made phantoms and experimental samples were imaged by CLE. Investigation of contrast of these images has been performed. With the help of histograms that presented CLE images, the contrast has been tightly investigated.