A. M. Taddese. Combining polarization gating imaging and hyperspectral analysis for biological tissues. Institut Fresnel, Karlsruhe School of Optics and Photonics; Institute of Biomedical Engineering, Karlsruhe Institute of Technology (KIT). Masterarbeit. 2019
Abstract:
Combining polarization and spectraly resolved information at the same time gives a good insight about the sample. While spectral map, in one side, helps to quantify chromophores, the polarization information, on the other side, gives structural information of the sam- ple with good path length selectivity. Two separate instruments are being used in the biomedical research. In this work, we have analyzed each method separately to show the feasibility of combining them for building a compact instrument. We have found that, ap- plication of polarization imaging improves the results of computing the spectral map, spe- cially in analyzing chromophores beneath the skin such as: oxy and deoxy hemoglobin. This has been veried rst by using dyes with known ratios, then with biological phantom and nally with in vivo measurements. We have also computed the maps of melanin for dark and white skin ngers and the results showed that a higher content is found in the dark one. Moreover, analysing the temporal change in absorbance of bacteria cultured in a petri dish veried to be useful for studying their growth rate whereas the polarization gating imaging helps in qualitative visualization of bacteria growth and division.